ABSTRACT: Fusarium oxysporum f. sp. lactucae (FOL) threatens lettuce production w orldw ide, yet genomic resources for many field isolates remain scarce, hampering both molecular race diagnostics and effector‑guided breeding. In this study, we produced the draft genome sequence of FOL isolate 16-086 collected from South Korea. High‑molecular‑weight DNA of the 16-086 extracted from a monoconidial culture was sequenced on the Illumina HiSeq X Ten platform (2 × 151 bp). De‑novo assembly with SOAPdenovo produced a 55.0 Mb genome distributed over 16,636 scaffolds (N₅₀ = 104 kb) with 1,941 bp of gaps and a GC content of 47.60 %. AUGUSTUS predicted 16,158 protein‑coding genes, 74.9 % of w hich carry recognisable InterPro domains, comparable to other F. oxysporum genomes. Whole‑genome completeness reached 96.3 % BUSCOs for Ascomycota. Pathogenicity was validated with the susceptible line ‘knou322’. Single‑locus PCR alone cannot conclusively assign strain 16‑086 to race 3; additional multi-loci assays and pathogenicity tests are required to resolve its race identity. The genome assembly and raw reads have been deposited in GenBank under BioProject PRJNA758594, BioSample SAMN21031248 and SRA SRR15671714, providing an open resource for refining molecular diagnostics and accelerating resistance‑gene deployment in lettuce breeding programmes.